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Cryosectioning PFA-fixed organoids were transferred to a cryomold, embedded in OCT compound (Thermo Fisher Scientific) and cooled to −80 °C in a bath of isopropanol on dry ice
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Because sickled red blood cells degrade more readily than healthy ones, a chronic deficiency of healthy red blood cells exists, which causes chronic anemia and exhaustion(Barua et al., 2023)
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